Opis stanowiska
Director of Centre of New Technologies of the University of Warsaw, with the Project Leader, announces opening of the position of Ph.D. Student in the Laboratory of Stem Cells, Development and Tissue Regeneration – Centre of New Technologies of the University of Warsaw.
Project title: Deciphering single-cell niche composition and their effect on hair follicle and dermal papillae Stem Cells regulation during the hair regeneration cycle
Project leader: Professor Krzysztof Kobielak
Competition number: CeNT-46-2023
For more informations, please visit the website of the University of Warsaw.
Nasze wymagania
- M.Sc. in Biology, Biotechnology or M.D. in Medicine
- Good knowledge of English
- Knowledge of Adobe Photoshop, Adobe Illustrator, PowerPoint
- Teamwork skills
- Experience or knowledge in laboratory work (gel electrophoresis, PCR, RT-PCR, q-PCR, DNA/RNA/Protein extraction/purification, DNA cloning, western blot, cryo- and paraffin- sectionings, cell culture, FACS sorting, immunofluorescent /immunohistochemistry staining, in situ hybridization, mouse breeding/numbering/genotyping, some basic mouse surgeries, microscope techniques)
- PhD student status (on the day of starting work in the project)
Zakres obowiązków
- A Ph.D. student will work with different genetically modified mice models to investigate molecular signaling between hair follicle Stem Cells (HFSCs) and niche components during hair follicles regeneration cycle. Thus, a Ph.D. student will be responsible to perform experiments on mice to induce HFSCs activation or quiescence. He/she will be able to investigate changes in surrounding niche components during hair cycle changes on the level of single-cell as well as address their molecular characteristics. Therefore, he/she will be involved in FACS sorting of "live" single-cell from whole skin (including stem cells), with subsequent single-cell isolation for barcoding for single-cell transcriptomics (scRNA-seq) with samples sequencing by next-generation sequencing (NGS). He/she will be also involved in building a proper platform for in situ single-cell assay. As a part of the regeneration assay, he/she will also perform grafting experiments of HFSCs with different niche components. He/she will be involved in tissue analysis (e.g. immunofluorescent staining) with the use of microscopy techniques
Dokumenty wymagane do rekrutacji
- Cover letter describing Candidate’s motivation
- Current curriculum vitae (CV), including information on known and used molecular biology techniques, a list of attended conferences (with titles and authors of the scientific presentations), publications, patents, scholarships, prizes and awards, or other relevant documents demonstrating the excellence of Candidate, Candidate's qualifications for the biology
- Master degree certificate (or equivalent)
- Document confirming PhD Student status (to be provided before starting work in the project)
- Letter of recommendation (LoR) from a scientist who is familiar with the Candidate (submitted directly to email address below)
- Information on the processing of personal data - the form is available at the CeNT UW webpage: https://cent.uw.edu.pl/en/wpcontent/uploads/sites/5/2020/07/Informationclause_personal-data-processing.pdf